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Time-resolved methods in Biophysics. 1. A novel pump and probe surface-enhanced resonance Raman approach for studying biological photoreceptors
Zitatschlüssel ISI:000243193700006
Autor Naumann, Hendrik and Klare, Johann P. and Engelhard, Martin and Hildebrandt, Peter and Murgida, Daniel H.
Seiten 1103-1108
Jahr 2006
ISSN 1474-905X
DOI 10.1039/b607807a
Adresse THOMAS GRAHAM HOUSE, SCIENCE PARK, MILTON RD, CAMBRIDGE CB4 0WF, CAMBS, ENGLAND
Journal Photochem. Photobiol. Sci.
Jahrgang 5
Nummer 12
Monat DEC
Verlag ROYAL SOC CHEMISTRY
Zusammenfassung This article describes a method, based on surface-enhanced resonance Raman (SERR) spectroscopy, for studying the reaction dynamics of photoreceptors immobilized on metal electrodes. Time resolution and fresh sample conditions are achieved by synchronizing the rotational and translational motion of a novel kinematic electrode with the duration and time delay between the pump and probe events. The power and sensitivity of the method is illustrated by studying the photocycle of the sensory photoreceptor NpSRII and its sensitivity to the applied electric field. The results are compared with time-resolved resonance Raman measurements in solution.
Typ der Publikation Review
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