TU Berlin

Physikalische Chemie / Biophysikalische ChemieArticles

Inhalt des Dokuments

zur Navigation

Articles

Effect of chromophore exchange on the resonance Raman spectra of recombinant phytochromes
Zitatschlüssel ISI:A1997XV23500005
Autor Kneip, C and Mozley, D and Hildebrandt, P and Gartner, W and Braslavsky, S E and Schaffner, K
Seiten 23-26
Jahr 1997
ISSN 0014-5793
Adresse PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS
Journal FEBS Lett.
Jahrgang 414
Nummer 1
Monat SEP 1
Verlag ELSEVIER SCIENCE BV
Zusammenfassung The recombinant 65-kDa polypeptide of phyA oat phytochrome was expressed by yeast Pichia pastor is and assembled into two chromopeptides with the chromophores phytochromobilin (P Phi B) and phycocyanobilin (PCB), respectively, The P-r and P-fr states of the two protein variants were characterized by resonance Raman (RR) spectroscopy and compared with native phyA oat phytochrome demonstrating that the deletion of the C-terminal half of phyA does not alter the structure of the chromophore site within the N-terminal half, Most of the RR spectral changes observed upon replacing P Phi B by PCB can be attributed exclusively to altered vibrational mode compositions due to the different ring D substitutions (vinyl vs, ethyl), implying that the chromophore structures are largely the same for P Phi B- and PCB-assembled phytochromes. Only in the P-r state may the RR spectral changes also reflect subtle differences of the P Phi B and PCB conformations in the 65-kDa phyA, presumably brought about by the specific steric requirements of the vinyl and ethyl groups. (C) 1997 Federation of European Biochemical Societies.
Typ der Publikation Article
Download Bibtex Eintrag

Navigation

Direktzugang

Schnellnavigation zur Seite über Nummerneingabe