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Comparison of the membrane-bound [NiFe] hydrogenases from R. eutropha H16 and D. vulgaris Miyazaki F in the oxidized ready state by pulsed EPR
Zitatschlüssel ISI:000274622900012
Autor Saggu, Miguel and Teutloff, Christian and Ludwig, Marcus and Brecht, Marc and Pandelia, Maria-Eirini and Lenz, Oliver and Friedrich, Baerbel and Lubitz, Wolfgang and Hildebrandt, Peter and Lendzian, Friedhelm and Bittl, Robert
Seiten 2139-2148
Jahr 2010
ISSN 1463-9076
DOI 10.1039/b922236g
Adresse THOMAS GRAHAM HOUSE, SCIENCE PARK, MILTON RD, CAMBRIDGE CB4 0WF, CAMBS, ENGLAND
Journal Phys. Chem. Chem. Phys.
Jahrgang 12
Nummer 9
Verlag ROYAL SOC CHEMISTRY
Zusammenfassung The geometric and electronic structures of the active sites in the oxidized Ni-r-B state of the [NiFe] hydrogenases from Ralstonia eutropha H16 and Desulfovibrio vulgaris Miyazaki F were investigated in pulsed EPR and ENDOR experiments at two different microwave frequencies (X- and Q-band). Two hyperfine-couplings were clearly resolved in the frozen solution spectra arising from the beta-protons of the nickel-coordinating cysteine residues Cys549 and Cys586 from the Desulfovibrio vulgaris and Ralstonia eutropha hydrogenase, respectively. ESEEM spectroscopic experiments reveal the presence of a histidine in the second coordination sphere of the Ni. The spectroscopic data indicate that the electronic structures of the [NiFe] centers in both hydrogenases are identical in the Ni-r-B state. However, additional spin couplings of the active site to further paramagnetic centers were identified for the Ralstonia eutropha hydrogenase. The respective couplings could be clearly resolved and simulated. The results from this study are discussed in view of the exceptional O-2-tolerance of the Ralstonia hydrogenase.
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