Inhalt des Dokuments
Articles
Zitatschlüssel | ISI:000088738000011 |
---|---|
Autor | Matysik, J and Hildebrandt, P and Ludwig, B |
Seiten | 125-130 |
Jahr | 2000 |
ISSN | 0005-2728 |
Adresse | PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS |
Journal | Biochim. Biophys. Acta |
Jahrgang | 1459 |
Nummer | 1 |
Monat | JUL 20 |
Verlag | ELSEVIER SCIENCE BV |
Zusammenfassung | Cytochrome-e oxidase aa(3) (CcO) from Paracoccus denitrificans interacts with tertiary butyl hydroperoxide (t-Bu-O-O-H, TBHP) by forming an adduct as indicated by an absorption shift at 408/432 nm and the induction of photochemical auto-reduction. The adduct was stable at room temperature for several days even under aerobic conditions. Upon irradiation (413 nm) of the adduct, a photoproduct, similar to the oxygenated mixed valence species (607 nm form), was formed, as indicated by the 418/442 and 607 nm signals in the absorption-difference spectrum. It is concluded that the adduct formation changes the photochemical properties of heme a(3). A molecular model for the binding mechanism of TBHP to CcO and for the photochemistry of heme a(3)-TBHP adduct is proposed. (C) 2000 Elsevier Science B.V. All rights reserved. |
Typ der Publikation | Article |
Zusatzinformationen / Extras
Direktzugang
Schnellnavigation zur Seite über Nummerneingabe
Hilfsfunktionen
Diese Seite verwendet Matomo für anonymisierte Webanalysen. Mehr Informationen und Opt-Out-Möglichkeiten unter Datenschutz.