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Concerted Action of Two Novel Auxiliary Proteins in Assembly of the Active Site in a Membrane-bound [NiFe] Hydrogenase
Zitatschlüssel ISI:000262545600025
Autor Ludwig, Marcus and Schubert, Torsten and Zebger, Ingo and Wisitruangsakul, Nattawadee and Saggu, Miguel and Strack, Angelika and Lenz, Oliver and Hildebrandt, Peter and Friedrich, Baerbel
Seiten 2159-2168
Jahr 2009
ISSN 0021-9258
DOI 10.1074/jbc.M808488200
Adresse 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3996 USA
Journal J. Biol. Chem.
Jahrgang 284
Nummer 4
Monat JAN 23
Zusammenfassung [NiFe] hydrogenases catalyze the reversible conversion of H-2 into protons and electrons. The reaction takes place at the active site, which is composed of a nickel and an iron atom and three diatomic ligands, two cyanides and one carbon monoxide, bound to the iron. The NiFe(CN-)(2)CO cofactor is synthesized by an intricate posttranslational maturation process, which is mediated by a set of six conserved Hyp proteins. Depending on the cellular location and the physiological function, additional auxiliary proteins are involved in hydrogenase biosynthesis. Here we present evidence that the auxiliary proteins HoxL and HoxV assist in assembly of the Fe(CN-)(2)CO moiety. This unit was identified as a cofactor intermediate of the oxygen-tolerant membrane-bound [NiFe] hydrogenase (MBH) in the beta-proteobacterium Ralstonia eutropha H16. Both HoxL and HoxV proved to be essential for H-2-oxidizing activity and MBH-driven growth on H-2. Copurification studies revealed that HoxL and HoxV directly interact with the hydrogenase apoprotein. HoxV forms complexes with HoxL and HypC, a HoxL paralogue that is essential for cofactor assembly. These observations suggest that HoxL acts as a specific chaperone assisting the transfer of the Fe(CN-)(2)CO cofactor intermediate from the Hyp machinery to the MBH. This shuttle also involves the scaffold protein HoxV. Indeed, infrared spectroscopy and metal analysis identified for the first time a non-redox-active Fe(CN-)(2)CO intermediate coordinated to HoxV.
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