direkt zum Inhalt springen

direkt zum Hauptnavigationsmenü

Sie sind hier

TU Berlin

Inhalt des Dokuments

Suche

Suche nach Publikationen




alle Publikationen (Reviews, Articles, Meetings and Proceedings)

Carbamoylphosphate serves as the source of CN-, but not of the intrinsic CO in the active site of the regulatory [NiFe]-hydrogenase from Ralstonia eutropha
Zitatschlüssel ISI:000248145800031
Autor Lenz, Oliver and Zebger, Ingo and Hamann, Josta and Hildebrandt, Peter and Friedrich, Baerbel
Seiten 3322-3326
Jahr 2007
ISSN 0014-5793
DOI 10.1016/j.febslet.2007.06.027
Adresse PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS
Journal FEBS Lett.
Jahrgang 581
Nummer 17
Monat JUL 10
Verlag ELSEVIER SCIENCE BV
Zusammenfassung Within the catalytic centre of [NiFe]-hydrogenases one carbonyl and two cyanide ligands are covalently attached to the iron. To identify the metabolic origins of these ligands, the regulatory [NiFe] hydrogenase in conjunction with the indigenous Hyp maturation proteins of Ralstonia eutropha H16 were heterologously overproduced in E. coli grown in the presence of L-[ureido-C-13] citrulline and (NaHCO3)-C-13. Infrared spectroscopy of purified hydrogenase provided direct evidence that only the cyanide ligands, but not the CO ligand, originate from CO2 and carbamoylphosphate. Incorporation of label from (CO)-C-13 exclusively into the carbonyl ligand indicates that free CO is a possible precursor in carbonyl ligand biosynthesis. (C) 2007 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
Typ der Publikation Article
Download Bibtex Eintrag

Zusatzinformationen / Extras

Direktzugang

Schnellnavigation zur Seite über Nummerneingabe

Diese Seite verwendet Matomo für anonymisierte Webanalysen. Mehr Informationen und Opt-Out-Möglichkeiten unter Datenschutz.