direkt zum Inhalt springen

direkt zum Hauptnavigationsmenü

Sie sind hier

TU Berlin

Inhalt des Dokuments

Suche

Suche nach Publikationen




alle Publikationen (Reviews, Articles, Meetings and Proceedings)

Switchable Redox Chemistry of the Hexameric Tyrosine-Coordinated Heme Protein
Zitatschlüssel ISI:000400534200006
Autor Kielb, Patrycja and Utesch, Tillmann and Kozuch, Jacek and Jeoung, Jae-Hun and Dobbek, Holger and Mroginski, Maria Andrea and Hildebrandt, Peter and Weidinger, Inez
Seiten 3955-3964
Jahr 2017
ISSN 1520-6106
DOI 10.1021/acs.jpcb.7b01286
Journal JOURNAL OF PHYSICAL CHEMISTRY B
Jahrgang 121
Nummer 16
Monat APR 27
Zusammenfassung Hexameric tyrosine-coordinated heme protein HTHP from Silicibacter pomeroyi has been shown to exhibit peroxidase- and catalase-like activity. In the present study, detailed spectroscopic and electrochemical investigations were performed to analyze the redox properties and active site structure of HTHP. Potentiometric titration of HTHP in solution revealed a single redox transition at -0.54 V (vs Ag/AgCl), indicating six structurally identical tyrosine coordinates hemes. Cyclic voltammetry (CV) of immobilized HTHP afforded a distinctly more positive redox potential (-0.17 V) but failed to detect a transition at -0.54 V. Conversely, surface enhanced RR (SERR) spectroscopy provided evidence for both high- and low-potential transitions and for a partial loss of heme in the reduced state. The high-potential CV-active redox transition is attributed to the hemes of the barrel-shaped HTHP in a wheel-like orientation on the surface. Supported by coarse-grained simulations and SERR spectroscopy, the majority of HTHP is concluded to adopt a reverse-disc orientation, accounting for the low-potential transition. In view of the striking similarity of HTHP to the heme carriers HasA or HmbR regarding redox potential, Fe-Tyr ligation, and heme release, we propose heme transport as an alternative or additional function.
Download Bibtex Eintrag

Zusatzinformationen / Extras

Direktzugang

Schnellnavigation zur Seite über Nummerneingabe

Diese Seite verwendet Matomo für anonymisierte Webanalysen. Mehr Informationen und Opt-Out-Möglichkeiten unter Datenschutz.