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Reversible Active Site Sulfoxygenation Can Explain the Oxygen Tolerance of a NAD(+)-Reducing [NiFe] Hydrogenase and Its Unusual Infrared Spectroscopic Properties
Citation key ISI:000350192700026
Author Horch, Marius and Lauterbach, Lars and Mroginski, Maria Andrea and Hildebrandt, Peter and Lenz, Oliver and Zebger, Ingo
Pages 2555-2564
Year 2015
ISSN 0002-7863
DOI 10.1021/ja511154y
Address 1155 16TH ST, NW, WASHINGTON, DC 20036 USA
Volume 137
Number 7
Month FEB 25
Abstract Oxygen-tolerant [NiFe] hydrogenases are metalloenzymes that represent valuable model systems for sustainable H-2 oxidation and production. The soluble NAD(+) reducing [NiFe] hydrogenase (SH) from Ralstonia eutropha couples the reversible cleavage of H-2 with the reduction of NAD(+) and displays a unique O-2 tolerance. Here we performed IR spectroscopic investigations on purified SH in various redox states in combination with density functional theory to provide structural insights into the catalytic [NiFe] center. These studies revealed a standard-like coordination of the active site with diatomic CO and cyanide ligands. The long-lasting discrepancy between spectroscopic data obtained in vitro and in vivo could be solved on the basis of reversible cysteine oxygenation in the fully oxidized state of the [NiFe] site. The data are consistent with a model in which the SH detoxifies O-2 catalytically by means of an NADH-dependent (per)oxidase reaction involving the intermediary formation of stable cysteine sulfenates. The occurrence of two catalytic activities, hydrogen conversion and oxygen reduction, at the same cofactor may inspire the design of novel biomimetic catalysts performing H-2-conversion even in the presence of O-2.
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