direkt zum Inhalt springen

direkt zum Hauptnavigationsmenü

Sie sind hier

TU Berlin

Inhalt des Dokuments

Publikationen

Substrate-Protein Interactions of Type II NADH:Quinone Oxidoreductase from Escherichia coli
Zitatschlüssel ISI:000376224000006
Autor Salewski, Johannes and Batista, Ana P. and Sena, Filipa V. and Millo, Diego and Zebger, Ingo and Pereira, Manuela M. and Hildebrandt, Peter
Seiten 2722-2734
Jahr 2016
ISSN 0006-2960
DOI 10.1021/acs.biochem.6b00070
Adresse 1155 16TH ST, NW, WASHINGTON, DC 20036 USA
Journal BIOCHEMISTRY
Jahrgang 55
Nummer 19
Monat MAY 17
Verlag AMER CHEMICAL SOC
Zusammenfassung Type II NADH:quinone oxidoreductases (NDH-2s) are membrane proteins involved in respiratory chains and responsible for the maintenance of NADH/NAD(+) balance in cells. NDH-2s are the only enzymes with NADH dehydrogenase activity present in the respiratory chain of many pathogens, and thus, they were proposed as suitable targets for antimicrobial therapies. In addition, NDH-2s were also considered key players for the treatment of complex I-related neurodegenerative disorders. In this work, we explored substrate protein interaction in NDH-2 from Escherichia coli (EcNDH-2) combining surface-enhanced infrared absorption spectroscopic studies with electrochemical experiments, fluorescence spectroscopy assays, and quantum chemical calculations. Because of the specific stabilization of substrate complexes of EcNDH-2 immobilized on electrodes, it was possible to demonstrate the presence of two distinct substrate binding sites for NADH and the quinone and to identify a bound semiprotonated quinol as a catalytic intermediate.
Typ der Publikation Article
Download Bibtex Eintrag

Zusatzinformationen / Extras

Direktzugang

Schnellnavigation zur Seite über Nummerneingabe

Diese Seite verwendet Matomo für anonymisierte Webanalysen. Mehr Informationen und Opt-Out-Möglichkeiten unter Datenschutz.