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Electrochemical and Infrared Spectroscopic Studies Provide Insight into Reactions of the NiFe Regulatory Hydrogenase from Ralstonia eutropha with O-2 and CO
Zitatschlüssel ISI:000363994000038
Autor Ash, Philip A. and Liu, Juan and Coutard, Nathan and Heidary, Nina and Horch, Marius and Gudim, Ingvild and Simler, Thomas and Zebger, Ingo and Lenz, Oliver and Vincent, Kylie A.
Seiten 13807-13815
Jahr 2015
ISSN 1520-6106
DOI 10.1021/acs.jpcb.5b04164
Adresse 1155 16TH ST, NW, WASHINGTON, DC 20036 USA
Journal JOURNAL OF PHYSICAL CHEMISTRY B
Jahrgang 119
Nummer 43
Monat OCT 29
Verlag AMER CHEMICAL SOC
Zusammenfassung The regulatory hydrogenase (RH) from Ralstonia eutropha acts as the H-2-sensing unit of a two-component system that regulates biosynthesis of the energy conserving hydrogenases of the organism according to the availability of H-2. The H-2 oxidation activity, which was so far determined in vitro with artificial electron acceptors, has been considered to be insensitive to O-2 and CO. It is assumed that bulky isoleucine and phenylalanine amino acid residues close to the NiFe active site ``gate'' gas access, preventing molecules larger than H-2 interacting with the active site. We have carried out sensitive electrochemical measurements to demonstrate that O-2 is in fact an inhibitor of H-2 oxidation by the RH, and that both H+ reduction and H-2 oxidation are inhibited by CO. Furthermore, we have demonstrated that the inhibitory effect of O-2 arises due to interaction of O-2 with the active site. Using protein film infrared electrochemistry (PFIRE) under H-2 oxidation conditions, in conjunction with solution infrared measurements, we have identified previously unreported oxidized inactive and catalytically active reduced states of the RH active site. These findings suggest that the RH has a rich active site chemistry similar to that of other NiFe hydrogenases.
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