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Raman spectroscopic and light-induced kinetic characterization of a recombinant phytochrome of the cyanobacterium Synechocystis
Citation key ISI:A1997YD64900032
Author Remberg, A and Lindner, I and Lamparter, T and Hughes, J and Kneip, C and Hildebrandt, P and Braslavsky, S E and Gartner, W and Schaffner, K
Pages 13389-13395
Year 1997
ISSN 0006-2960
Address 1155 16TH ST, NW, WASHINGTON, DC 20036
Journal Biochemistry
Volume 36
Number 43
Month OCT 28
Abstract A phytochrome-encoding cDNA from the cyanobacterium Synechocystis has been heterologously expressed in Escherichia coli and reconstituted into functional chromoproteins by incubation with either phycocyanobilin (PCB) or phytochromobilin (P Phi B). These materials were studied by Raman spectroscopy and nanosecond flash photolysis. The Raman spectra suggest far-reaching, similarities in chromophore configuration and conformation between the P-fr forms of Synechocystis phytochrome and the plant phytochromes (e.g. phyA from oat), but some differences, such as torsions around methine bridges and in hydrogen bonding interactions, in the P-r, state. Synechocystis phytochrome (PCB) undergoes a multistep photoconversion reminiscent of the phyA P-r–>P-fr transformation but with different kinetics. The first process resolved is the decay of an intermediate with red-shifted absorption (relative to parent state) and a 25-mu s lifetime. The next observable intermediate grows in with 300 (+/-25) mu s and decays with 6-8 ms, The final state (P-fr) is formed biexponentially (450 ms, 1 s). When reconstituted with P Phi B, the first decay of this Synechocystis phytochrome is biexponential (5 and 25 mu s). The growth of the second intermediate is slower (750 mu s) than that in the PCB adduct whereas the decays of both species are similar. The formation of the P-fr form required fitting with three components (350 ms, 2.5 a, and 11 s). H/D Exchange in Synechocystis phytochrome (PCB) delays, by an isotope effect of 2.7, both growth (300 mu s) and decay rates (6-8 ms) of the second intermediate. This effect is larger than values determined for phyA (ca. 1.2) and is characteristic of a rate-limiting proton transfer. The formation of the Pc, state of the PCB adduct of Synechocystis phytochrome shows a deuterium effect similar as phyA ten. 1.2). Activation energies of the second intermediate in the range 0-18 degrees C are 44 (in H2O/buffer) and 48 kJ mol(-1) (D2O), with essentially identical pre-exponential factors.
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