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Citation key | ISI:000248145800031 |
---|---|
Author | Lenz, Oliver and Zebger, Ingo and Hamann, Josta and Hildebrandt, Peter and Friedrich, Baerbel |
Pages | 3322-3326 |
Year | 2007 |
ISSN | 0014-5793 |
DOI | 10.1016/j.febslet.2007.06.027 |
Address | PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS |
Journal | FEBS Lett. |
Volume | 581 |
Number | 17 |
Month | JUL 10 |
Publisher | ELSEVIER SCIENCE BV |
Abstract | Within the catalytic centre of [NiFe]-hydrogenases one carbonyl and two cyanide ligands are covalently attached to the iron. To identify the metabolic origins of these ligands, the regulatory [NiFe] hydrogenase in conjunction with the indigenous Hyp maturation proteins of Ralstonia eutropha H16 were heterologously overproduced in E. coli grown in the presence of L-[ureido-C-13] citrulline and (NaHCO3)-C-13. Infrared spectroscopy of purified hydrogenase provided direct evidence that only the cyanide ligands, but not the CO ligand, originate from CO2 and carbamoylphosphate. Incorporation of label from (CO)-C-13 exclusively into the carbonyl ligand indicates that free CO is a possible precursor in carbonyl ligand biosynthesis. (C) 2007 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved. |
Bibtex Type of Publication | Article |
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