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Resonance Raman spectroscopic study of the tryptic 39-kDa fragment of phytochrome
Citation key ISI:000089847300017
Author Kneip, C and Schlamann, W and Braslavsky, S E and Hildebrandt, P and Schaffner, K
Pages 252-256
Year 2000
ISSN 0014-5793
Address PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS
Journal FEBS Lett.
Volume 482
Number 3
Month OCT 6
Publisher ELSEVIER SCIENCE BV
Abstract The 39-kDa fragment of oat phytochrome phyA, obtained by tryptic digestion at the amino acids 65 and 425, was studied by resonance Raman spectroscopy. The parent state P-r reveals far-reaching similarities with that of the native phytochrome implying that the structures of the tetrapyrrole chromophore and its immediate protein environment are not affected by the proteolysis. However, the resonance Raman spectrum of the final product of the P-r phototransformation, denoted as P-bl, is more closely related to that of the P-fr precursor of the native phytochrome, i.e. meta-R-C, rather than to that of P-fr itself. The resonance Raman spectra indicate a high conformational flexibility of the chromophore in P-bl so that, unlike in P-fr, the tetrapyrrole rings C and D adopt a largely coplanar conformation. The protein interactions with ring D of the chromophore, which in the native phytochrome stabilize the specific chromophore structure of P-fr, cannot be established in the 39-kDa fragment due to the lack of the major C-terminal part of the protein. These findings, furthermore, support the view that the meta-R-C –>P-fr transition is associated with a coupling of chromophore and protein structural changes that represent crucial events for the photoactivation of phytochrome. (C) 2000 Federation of European Biochemical Societies. Published by Elsevier Science B.V. All rights reserved.
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