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| Citation key | ISI:A1992JL50200029 |
|---|---|
| Author | Hildebrandt, P and Hoffmann, A and Lindemann, P and Heibel, G And Braslavsky, S E and Schaffner, K and Schrader, B |
| Pages | 7957-7962 |
| Year | 1992 |
| ISSN | 0006-2960 |
| Address | 1155 16TH ST, NW, WASHINGTON, DC 20036 |
| Journal | Biochemistry |
| Volume | 31 |
| Number | 34 |
| Month | SEP 1 |
| Publisher | AMER CHEMICAL SOC |
| Abstract | The P(r) and P(fr) forms of phytochrome in H2O and D2O have been studied by Fourier transform resonance Raman spectroscopy with near-infrared excitation (1064 nm). It is demonstrated that this technique is a powerful method for analyzing the chromophore structures of photosensitive pigments. The high spectral quality allows discussion of vibrational assignments based on an empirical approach using previously published data obtained from model compounds. The reduction in intensity of a high-frequency band assigned to the ring-C/D methine bridge vibration is an indication for the non-coplanarity of the ring D in P(fr). The high intensity of a C-H out-of-plane vibration also supports this hypothesis. In P(r), a broad peak at approximately 1100 cm-1 is assigned to an out-of-planevibration of a strongly hydrogen-bonded pyrrole C=NH+ group. It is missing in P(fr), suggesting deprotonation of the corresponding ring during the transformation from P(r) to P(fr). |
| Bibtex Type of Publication | Article |
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