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Fourier-transform resonance Raman-spectroscopy of phytochrome
Citation key ISI:A1992JL50200029
Author Hildebrandt, P and Hoffmann, A and Lindemann, P and Heibel, G And Braslavsky, S E and Schaffner, K and Schrader, B
Pages 7957-7962
Year 1992
ISSN 0006-2960
Address 1155 16TH ST, NW, WASHINGTON, DC 20036
Journal Biochemistry
Volume 31
Number 34
Month SEP 1
Publisher AMER CHEMICAL SOC
Abstract The P(r) and P(fr) forms of phytochrome in H2O and D2O have been studied by Fourier transform resonance Raman spectroscopy with near-infrared excitation (1064 nm). It is demonstrated that this technique is a powerful method for analyzing the chromophore structures of photosensitive pigments. The high spectral quality allows discussion of vibrational assignments based on an empirical approach using previously published data obtained from model compounds. The reduction in intensity of a high-frequency band assigned to the ring-C/D methine bridge vibration is an indication for the non-coplanarity of the ring D in P(fr). The high intensity of a C-H out-of-plane vibration also supports this hypothesis. In P(r), a broad peak at approximately 1100 cm-1 is assigned to an out-of-planevibration of a strongly hydrogen-bonded pyrrole C=NH+ group. It is missing in P(fr), suggesting deprotonation of the corresponding ring during the transformation from P(r) to P(fr).
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