direkt zum Inhalt springen

direkt zum Hauptnavigationsmenü

Sie sind hier

TU Berlin

Page Content


Search for publications

all Publications (Reviews, Articles, Meetings and Proceedings)

The Fungal Phytochrome FphA from Aspergillus nidulans
Citation key ISI:000261469100015
Author Brandt, Sonja and von Stetten, David and G�nther, Mina and Hildebrandt, Peter and Frankenberg-Dinkel, Nicole
Pages 34605-34614
Year 2008
ISSN 0021-9258
DOI 10.1074/jbc.M805506200
Address 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3996 USA
Journal J. Biol. Chem.
Volume 283
Number 50
Month DEC 12
Abstract The red light-sensing photoreceptor FphA from Aspergillus nidulans is involved in the regulation of developmental processes in response to light. Here we present extended biochemical and spectroscopic characterization of recombinant FphA using a synthetic gene with host-adapted codon usage. The recombinant photosensory domain FphAN753 was shown to display all features of a bona fide phytochrome. It covalently binds biliverdin as chromophore and undergoes red/far-red light-inducible photoconversion with both parent states being protonated. The large N-terminal variable extension of FphA exerts a stabilizing effect on the active Pfr state. Upon substitution of the highly conserved histidine 504, involved in the hydrogen-bonding network of the protein moiety and the chromophore, chromophore attachment and photoreversibility were completely impaired. FphA is a functional sensor histidine kinase with a strong red-light-dependent autophosphorylation activity. Furthermore, intermolecular trans-phosphorylation to the response regulator domain of a second monomer could be demonstrated. Interestingly, co-incubation of FphA and FphA variants led to enhanced autophosphorylation, including the ``inactive'' Pr form. The latter observed phenomenon might suggest that auto- and trans-phosphorylation activity is modulated by additional interaction partners leading to variable phosphorylation events that trigger a specific output response.
Bibtex Type of Publication Article
Download Bibtex entry

Zusatzinformationen / Extras

Quick Access:

Schnellnavigation zur Seite über Nummerneingabe

This site uses Matomo for anonymized webanalysis. Visit Data Privacy for more information and opt-out options.