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Analysis of vibrational spectra of multicomponent systems. Application to pH-dependent resonance Raman spectra of ferricytochrome c
Citation key ISI:A1996UN50400011
Author Dopner, S and Hildebrandt, P and Mauk, A G and Lenk, H and Stempfle, W
Pages 573-584
Year 1996
ISSN 0584-8539
Address THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD, ENGLAND OX5 1GB
Journal Spectrochimica Acta Part A-Molecular and Biomolecular Spectroscopy
Volume 52
Number 5
Month MAY
Publisher PERGAMON-ELSEVIER SCIENCE LTD
Abstract A method is presented which is appropriate to analyse complex vibrational spectra of molecular systems including several components. The method which is related to global analysis is directed to determine the spectra of the individual components together with their relative contributions in a given set of vibrational spectra. The advantages of this approach are investigated by systematically analysing a series of artificially generated spectra, The applicability of this method to the analysis of experimental spectra is demonstrated in a resonance Raman study of a pH-dependent conformational equilibrium of cytochrome c.
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